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Amplitaq Gold Polymerase. The ‘gold standard’ method to determine the level of somatic activity is by polymerase chain reaction (pcr) of the target region followed by cloning and sequencing of a large number of clones (21,22). Sequencing primer and polymerase were added to the final enriched spheres before loading onto an ion 318 chip according to the ion pgmtm 200 sequencing kit protocol.
When amplitaq gold™ 360 pcr master mix is added to the reaction mixture at room temperature, the inactive enzyme is not capable of primer extension. As the reaction progresses, the 5′ vic® dye is cleaved by the 5′ nuclease activity of amplitaq gold polymerase, thereby separating it from the quencher. The ‘gold standard’ method to determine the level of somatic activity is by polymerase chain reaction (pcr) of the target region followed by cloning and sequencing of a large number of clones (21,22).
Sequencing Primer And Polymerase Were Added To The Final Enriched Spheres Before Loading Onto An Ion 318 Chip According To The Ion Pgmtm 200 Sequencing Kit Protocol.
The ‘gold standard’ method to determine the level of somatic activity is by polymerase chain reaction (pcr) of the target region followed by cloning and sequencing of a large number of clones (21,22). The fluorescence of the released dye per cycle is a measure of the resultant products. This reaction is possible only if both the sense primer and the probe primer anneal on the same strand.
As The Reaction Progresses, The 5′ Vic® Dye Is Cleaved By The 5′ Nuclease Activity Of Amplitaq Gold Polymerase, Thereby Separating It From The Quencher.
When amplitaq gold™ 360 pcr master mix is added to the reaction mixture at room temperature, the inactive enzyme is not capable of primer extension.
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